Elisa plate,1 box 96-well ELISA plate, OEM manufacturer, medical injection products

Elisa plate,1 box 96-well ELISA plate, OEM manufacturer, medical injection products

Enzyme-linked immunosorbent assay (ELISA) is a widely used technique in biomedical research and diagnostics for detecting the presence of specific proteins, antibodies, or antigens in biological samples. The ELISA process involves several key components, including the microplate, which plays a crucial role in facilitating the immunological reactions.

Microplates used in ELISA experiments are typically made of polystyrene (PS) material, chosen for its optical clarity, inertness, and compatibility with various detection methods. These plates are designed specifically for ELISA applications, with dimensions that adhere to the Society for Biomolecular Screening (SBS) international standards. The well-structured layout of the microplate allows for efficient sample handling and analysis, ensuring reproducibility and accuracy in experimental results.

In an ELISA experiment, the purity, concentration, and ratio of the antigens, antibodies, and labeled antibodies or antigens are critical factors influencing the sensitivity and specificity of the assay. The choice of buffer solution, its concentration, ionic strength, pH (expressed as the DH value), and reaction temperature and duration are also pivotal in determining the success of the immunoassay.

The antigen-antibody interactions on the microplate surface are highly dependent on the quality of the surface coating and the blocking reagents used to prevent non-specific binding. Proper washing steps between incubation periods are essential to remove unbound molecules and reduce background noise, enhancing the signal-to-noise ratio of the assay.